Aminoacid Sequence of Dogfish M4 Lactate Dehydrogenase
نویسندگان
چکیده
منابع مشابه
The amino acid sequence of the tryptic peptides isolated from dogfish M4 lactate dehydrogenase.
The peptides which result from treatment of the S-[14C]carboxymethyl derivative of dogfish M4 lactate dehydrogenase (EC 1.1.1.27) with trypsin have been isolated and their sequences have been elucidated. Each identical subunit has a molecular weight of 36,000 and on the basis of the amino acid composition 40 unique tryptic peptides are anticipated. Thirty-seven of these peptides have been isola...
متن کاملThe subunits of dogfish M4 lactic dehydrogenase.
Treatment of dogfish l& lactic dehydrogenase with carboxypeptidase A releases 1 mole of phenylalanine per 36,000 g of the enzyme and no other amino acids. A mixture of carboxypeptidases A and B releases 1 mole of phenylalanine and 1 mole of lysine per 36,000 g of the lactic dehydrogenase. The amino terminus of dogfish M4 lactic dehydrogenase does not react with either dansyl chloride or the Edm...
متن کاملAmino acid sequence of dogfish muscle lactate dehydrogenase.
Lactate dehydrogenase isolated from vertebrate tissues catalyzes the reversible oxidation of L-lactic acid to pyruvic acid. The catalysis requires nicotinamide adenine dinucleotide (NAD’) as a coenzyme and is specific for the A-hydrogen of the nicotinamide ring (1) (Fig. 1). Although the enzyme is tetrameric, no cooperative effects have been observed between subunits (2). Each identical subunit...
متن کاملSynthesis and Degradation of Rat Liver Lactate Dehydrogenase M4 HYBRIDIZATION IN THE PURIFICATION OF LACTATE DEHYDROGENASE
For the measurement of lactate dehydrogenase (LDH) isoenzyme biosynthesis a new method is presented which is based on the quantitative hybridization of isoenzyme MI with isoenzyme Hq. After reversible dissociation the formation of active LDH M4 was more rapid and occurred to a greater extent than the formation of LDH Hq. The formation of hybrid isoenzymes from the two subunits resulted in an in...
متن کاملSynthesis and degradation of rat liver lactate dehydrogenase M4. Hybridization in the purification of lactate dehydrogenase isoenzymes.
For the measurement of lactate dehydrogenase (LDH) isoenzyme biosynthesis a new method is presented which is based on the quantitative hybridization of isoenzyme MI with isoenzyme Hq. After reversible dissociation the formation of active LDH M4 was more rapid and occurred to a greater extent than the formation of LDH Hq. The formation of hybrid isoenzymes from the two subunits resulted in an in...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1973
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.70.6.1790